Transmission spectra for the dry and moist biotic scenarios 1 and 2. We show the JWST/NIRSpec range between 3 and 5 µm (top) with an inlay for the 3.2–3.35 µm range to highlight the O₃ (grey box) and CH₄ feature (magenta box). The bottom panel shows transmission spectra for 5–10 µm in the JWST/MIRI range (bottom). We note that the transmission spectra for the dry biotic scenarios 1 and 2 are identical. We further simulated JWST observations with PANDEXO for one scenario (dry 1/2) with 100 transit observations of TRAPPIST-1e (black error bars). We chose the G395M grism setting with 10 pixels per bin (top), 10 and 20 pixels per bins (inlay), and MIRI LRS setting with 5 pixels per bin (bottom panel).