Processing of the NanoRocks data frames for statistical data analysis. (a) The gray frame consists of a mixture of the three(red, green and blue) layers of the original frame, chosen to distinctly reveal the red and blue beads. (b) This averaged frame is produced by averaging 10 consecutive gray frames. (c) The next step consists in subtracting each frame from its following frame. (d) Finally, the subtracted frame gets despeckled and binarized.