Example charge trails from three epochs of Gaia in-orbit serial CTI calibration activities. The mean level of the charge injection in the image section CCD columns is shown before the start of the post-scan measurements illustrating the change of two orders of magnitude in the commanded blocks. A small increase in serial CTI is observed from early to late epochs in the mission time line, particularly for the release signature at longer timescales. These data come from device AF5 in row 5 near the middle of the focal plane array.